Methods for Directed Exaptation

A method for producing a treatment for a Multiple-Antibiotic Resistant Infection, comprising the steps of:

• creating an antiMARI microorganism by the steps of :
• dividing an original population comprising a monoculture of a plurality of microorganisms into a plurality of subcultures having initially identical composition;
• subjecting at least some of the plurality of subcultures to a plurality of different exaptation agents to obtain diversified substrains of the microorganism, wherein the exaptation agents are selected to favor survival of sub strains, or sets of substrains, wherein at least a first portion of the subcultures is subjected to different types or combinations of exaptation agents than are subcultures in other portions, and wherein at least one of the exaptation agents is selected to favor survival of substrains of the microorganism that exhibiting an ability to outcompete the Multiple-Antibiotic Resistant Infection;
• optionally iterating the steps of dividing and subjecting, using at least a subset of the diversified substrains in place of the original population, until an ability to outcompete the Multiple-Antibiotic Resistant Infection is achieved;
• and
• combining at least some of the diversified substrains with microorganisms from the original monoculture to create the Multiple-Antibiotic Resistant Infection treatment.