Sample preparation and microbial analysis
AssigneeMiraki Innovation Think Tank LLC
InventorsJames HILL, Nisha Veronica VARMA, Gregory T. MARTIN, Goossen Jan Bernard BOER, Zhiqian ZHOU, Christopher VELIS
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Abstract
Methods are described for preparing samples including biological, environmental, and food products for microbial analysis. Microbes and microbe components in the sample can be treated with antimicrobial compounds and a matrix solution to permit fast and accurate characterization using analysis techniques such as matrix-assisted laser desorption/ionization Time-of-Flight mass spectrometry (MALDI-TOF MS).
Claims59
A method of preparing a sample for detecting a microbe or microbe components present in the sample, the method comprising the following steps:
- adding a substance to a sample suspected of comprising a microbe or microbe components;
- digesting the sample under conditions promoting digestion of a microbe or microbe components in the sample;
- and
- optionally contacting the digested microbe or microbe components with a matrix or matrix solution on a target substrate.
The method according to 53, further comprising identifying the antimicrobial susceptibility of the pathogen.
A method of preparing a sample for detecting a microbe or microbe components present in the sample, the method comprising the following steps:
- isolating from a sample a microbe or microbe components bound to a microbe-targeting molecule on a substrate;
- adding a substance to the isolated microbe or microbe components;
- digesting the isolated microbe or microbe components under conditions promoting digestion of the microbe or microbe components;
- and
- contacting the digested microbe or microbe components with a matrix or matrix solution on a target substrate.
Dependencies are inferred from the claim text.