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US 2023/0054471 A1Published application
Long poly(a) plasmids and methods for introduction of long poly(a) sequences into the plasmid
AssigneeSeattle Children's Hospital (dba Seattle Children's Research Institute)
InventorsAndrew M. Scharenberg, Kyle Jacoby, Alexandra E. Grier
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Abstract
Disclosed herein are polynucleotides having a plurality of thymine nucleotides and an endonuclease recognition site inserted therein, methods of engineering the polynucleotides having a plurality of thymine nucleotides and an endonuclease recognition site inserted therein, and methods of enhancing transcription, translation, and increasing stability of a polynucleotide.
Claims21
(canceled)
An isolated polynucleotide encoding a polypeptide, the polynucleotide comprising:
- (a) a first nucleic acid encoding the polypeptide;
- and
- (b) a second nucleic acid comprising a plurality of thymine nucleotides of at least 130 consecutive thymine nucleotides, and an endonuclease cleavage site located within the plurality of thymine nucleotides or within 40 consecutive nucleotides from the 5′ terminal thymine of the plurality of thymine nucleotides, and
- (c) a third nucleic acid comprising an endonuclease recognition site, wherein the endonuclease recognition site is within 55 consecutive nucleotides of the endonuclease cleavage site, wherein the second nucleic acid is covalently linked to the third nucleic acid at one end of the third nucleic acid.
1 more element
Dependencies are inferred from the claim text.