Double-stranded dna molecule for the detecting and characterizing molecular interactions
AssigneesParis Sciences Et Lettres—Quartier Latin, Centre National de la Recherche Scientifique (CNRS), Ecole Normale Superieure, Universite De Paris
InventorsTérence STRICK, Charlie GOSSE, Dorota KOSTRZ, Jinglong WANG, Marc NADAL
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Abstract
The present application relates to a double-stranded DNA molecule comprising a first double-stranded DNA molecule (1) connected to a second double-stranded DNA molecule (2) by at least one covalent bond which is not a phosphodiester, phosphorothioate, phosphoramidate or phosphorodiamidate bond, preferably by a tether, said tether preferably being a double-stranded DNA molecule.
Claims22
A double-stranded DNA molecule comprising a first double-stranded DNA molecule (1) connected to a second double-stranded DNA molecule (2) by a tether comprising double-stranded DNA, wherein the tether is attached by
- (i) at least one covalent bond to a nucleotide of the first double-stranded DNA molecule (1), and by
- (ii) at least one covalent bond to a nucleotide of the second double-stranded DNA molecule (2),
- wherein
- the at least one covalent bond of (i) and the at least one covalent bond of (ii) are not phosphodiester bonds, phosphorothioate bonds, phosphoramidate bonds or phosphorodiamidate bonds, and
- the nucleotide of (i) and the nucleotide of (ii) are not the ultimate nucleotides of double-stranded DNA molecules (1) and (2).
(canceled)
A double-stranded DNA molecule comprising a first double-stranded DNA molecule (A) and a second double-stranded DNA molecule (B), wherein
- the first double-stranded DNA molecule (A) comprises a cleavage site which is present only in the first double-stranded DNA molecule (A),
- the first double-stranded DNA molecule (A) is connected to the second double-stranded DNA molecule (B) by two covalent bonds which are not phosphodiester bonds, phosphorothioate bonds, phosphoramidate bonds or phosphorodiamidate bonds, and
- one of the two covalent bonds is located on each side of the cleavage site.
(canceled)
Dependencies are inferred from the claim text.