Back to results
US 2023/0053493 A1Published application
One step methods, kits, and systems for the measurement of concentrations of unbound bilirubin in biological fluids
OwnerAlan Marc Kleinfeld, Andrew H. Huber +3inventors
InventorsAlan Marc Kleinfeld, Andrew H. Huber, Hiroto Kameyama, Tung-Chih Sun, Divya Subramonian
Life of this patent
Estimating the term…
Abstract
Identification and use of proteins fluorescently labeled and that undergo a change in fluorescence index upon binding bilirubin are described. Probes are disclosed which are labeled at a cysteine or lysine residue and also probes labeled at both cysteine and lysine with two different fluorophores. These probes are useful for determination of unbound bilirubin levels in a fluid sample.
Claims30
A sensor for measuring free bilirubin in a sample, the sensor comprising:
- a bilirubin responsive probe labeled with a first fluorophore;
- and
- a non-responder probe labeled with a second fluorophore,
- wherein the first and second fluorophores excite at the same wavelength, and
- wherein the first and second fluorophore emit a fluorescence at different wavelengths.
2 more elements
20-24. (canceled)
34-58. (canceled)
Dependencies are inferred from the claim text.