Method and system for dna detection
AssigneeHitachi High-Tech Corporation
InventorsJunko TANAKA, Tatsuo NAKAGAWA, Akiko SHIRATORI, Chihiro UEMATSU
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AssigneeHitachi High-Tech Corporation
InventorsJunko TANAKA, Tatsuo NAKAGAWA, Akiko SHIRATORI, Chihiro UEMATSU
Estimating the term…
Provided are a method and a system for accurately distinguishing a micropartition in which a target gene or target DNA is placed and a micropartition in which a pseudogene is placed by a measuring device and accurately counting the target gene or target DNA in digital PCR using melting curve analysis. The method includes the steps of: placing a DNA solution in each of a plurality of partitions; performing PCR; changing a temperature of each of the partitions and measuring a fluorescence intensity; calculating a melting temperature of a double strand DNA for each of the partitions; counting the number of partitions for each type of the DNA; outputting the number of partitions counted for each type of the DNA; and discriminating the first gene and the pseudogene based on the melting temperature and the number of the counted partitions.
A DNA detection method comprising the steps of:
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A DNA detection system comprising:
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Dependencies are inferred from the claim text.