CCA Gene For Virus Resistance
OwnerJonathan KALISVAART, Raoul Jacobus Johannes Maria FRIJTERS +2inventors
InventorsJonathan KALISVAART, Raoul Jacobus Johannes Maria FRIJTERS, Daniël Johannes Wilhelmus LUDEKING, Alwin Johannes Marinus ROOVERS
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Abstract
The present invention relates to a modified CCA gene which encodes a CCA-adding enzyme, which modified CCA gene leads to resistance against a positive-strand RNA virus having a transfer RNA-like structure (TLS). The invention further relates to plants and seeds comprising the modified genes, methods for making and identifying such plants and use of the gene.
Claims30
A modified CCA gene which encodes a CCA-adding enzyme, which modified CCA gene leads to resistance against a positive-strand RNA virus having a TLS, wherein the modified CCA gene is selected from the group consisting of:
- a gene comprising a nucleotide sequence that encodes a CCA-adding enzyme according to SEQ ID NO:
- 8, SEQ ID NO:
- 9, SEQ ID NO:
- 10, or SEQ ID NO:
- 11;
- a gene comprising a promoter sequence comprising SEQ ID NO:
- 12, SEQ ID NO:
- 13, SEQ ID NO:
- 14, SEQ ID NO:
- 15, or SEQ ID NO:
- 16;
- a gene comprising a nucleotide sequence that encodes a CCA-adding enzyme having a deletion, a substitution, or an insertion of at least one amino acid when compared to SEQ ID NO:
- 2 or SEQ ID NO:
- 7;
- a gene comprising a nucleotide sequence that encodes a CCA-adding enzyme having a deletion, a substitution, or an insertion of at least one amino acid when compared to SEQ ID NO:
- 8, SEQ ID NO:
- 9, SEQ ID NO:
- 10, or SEQ ID NO:
- 11;
- a gene comprising a nucleotide sequence that encodes a CCA-adding enzyme having at least 80% sequence identity to SEQ ID NO:
- 8, SEQ ID NO:
- 9, SEQ ID NO:
- 10, or SEQ ID NO:
- 11;
- and
- a gene comprising a promoter sequence having at least 80% sequence identity to SEQ ID NO:
- 12, SEQ ID NO:
- 13, SEQ ID NO:
- 14, SEQ ID NO:
- 15, or SEQ ID NO:
- 16.
A marker for identifying a modified CCA gene, wherein the marker detects a modification selected from the group consisting of:
- an A to T SNP on position 948 of SEQ ID NO:
- 1,
- a C to T SNP on position 950 of SEQ ID NO:
- 1,
- an A to G SNP on position 1348 of SEQ ID NO:
- 1,
- an A to G SNP on position 1603 of SEQ ID NO:
- 1,
- an A to T SNP on position 1659 of SEQ ID NO:
- 1,
- a G to T SNP on position 1737 of SEQ ID NO:
- 1,
- a T to C SNP on position 631 of SEQ ID NO:
- 5, and
- a deletion in SEQ ID NO:
- 3 comprising SEQ ID NO:
- 4,
- or wherein the marker detects a modification on a corresponding position of a homologous sequence having at least 80% sequence identity to SEQ ID NO:
- 1 or SEQ ID NO:
- 3 or SEQ ID NO:
- 5.
A method for selecting a ToBRFV resistant Solanum lycopersicum plant, comprising identifying the presence of a modification in a CCA gene, optionally testing the plant for ToBRFV resistance, and selecting a plant that comprises said modification as a ToBRFV resistant plant.
Dependencies are inferred from the claim text.