Chimeric filovirus vaccines

A polynucleotide comprising a sequence of a live, infectious, attenuated Flavivirus, the polynucleotide comprising:

• a nucleotide sequence that encodes at least a part of a Filovirus glycoprotein lacking an N terminal signal peptide,
• wherein:
• the nucleotide sequence is located at an intergenic region between an E gene of the Flavivirus and an NS1 gene of the Flavivirus, the E gene encoding an E protein, the NS1 gene encoding an NS1 protein, the NS1 protein comprising a signal peptide, such that a chimeric virus is expressed;
• and
• the nucleotide sequence is translatable such that the nucleotide sequence encodes a chimeric viral peptide, the chimeric viral peptide comprising:
• (a) a further signal peptide, positioned C terminally of the E protein, the further signal peptide having the same sequence as the signal peptide of the NS1 protein,
• (b) the Filovirus glycoprotein, positioned C terminally of the further signal peptide, and
• (c) a TM domain of a further Flaviviral E protein, positioned C terminally of the Filovirus glycoprotein;
• and
• the chimeric viral peptide is positioned N terminally of the NS1 protein of the Flavivirus.

A chimeric live, infectious, attenuated Flavivirus comprising:

• an E protein;
• an NS1 protein comprising a signal peptide;
• and
• a chimeric protein comprising:
• at least a part of a Filovirus glycoprotein lacking a functional signal peptide, wherein the Filovirus glycoprotein is inserted between the E protein and the NS1 protein;
• a further signal peptide, positioned C terminally of the E protein, wherein the further signal peptide has the same sequence as the signal peptide of the NS1 protein, and wherein the Filovirus glycoprotein is positioned C terminally of the further signal peptide;
• and
• a TM domain of a further Flaviviral E protein, positioned C terminally of the Filovirus glycoprotein,
• wherein the chimeric peptide is positioned N terminally of the NS1 protein.