Antimicrobial, bacteriophage-derived polypeptides and their use against gram-negative and acid-fast bacteria

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A method of inhibiting the growth, reducing the population, or killing of at least one species of Gram-negative bacteria, the method comprising contacting the bacteria with a composition comprising an effective amount of (i) a Chp peptide comprising an amino acid sequence selected from the group consisting of SEQ ID NOs. 81-91 and 94-102 or active fragments thereof, or (ii) a modified Chp peptide having 80% sequence identity with the amino acid sequence of at least one of SEQ ID NOs. 81-91 and 94-102, said Chp peptide or modified Chp peptide having lytic activity for a period of time sufficient to inhibit said growth, reduce said population, or kill said at least one species of Gram-negative bacteria.

A method of inhibiting the growth, reducing the population, or killing of at least one species of acid-fast bacteria, the method comprising contacting the bacteria with a composition comprising an effective amount of (i) a Chp peptide comprising an amino acid sequence selected from the group consisting of SEQ ID NOs:

• 1, 2, 4, 6, 8-21, 23-26, 59-61, 63-65, 67, 81, 87-89, 91, 97, and 99-101 or active fragments thereof, or (ii) a modified Chp peptide having 80% sequence identity with the amino acid sequence of at least one of SEQ ID NOs. 1, 2, 4, 6, 8-21, 23-26, 59-61, 63-65, 67, 81, 87-89, 91, 97, and 99-101, said Chp peptide or modified Chp peptide having lytic activity for a period of time sufficient to inhibit said growth, reduce said population, or kill said at least one species of acid-fast bacteria.

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A method for prevention, disruption, or eradication of a biofilm comprising at least one species of Gram-negative bacteria, the method comprising contacting a biofilm with a pharmaceutical composition comprising (i) an isolated Chp peptide having an amino acid sequence selected from the group consisting of SEQ ID NOs. 81-91 and 94-102 or active fragment thereof, or (ii) a modified Chp peptide having 80% sequence identity with the amino acid sequence of at least one of SEQ ID NOs. 81-91 and 94-102, wherein the modified Chp peptide inhibits the growth, reduces the population, or kills at least one species of Gram-negative bacteria, and wherein the biofilm is effectively prevented, dispersed, or eradicated.