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AssigneeShanghai Ocean University
InventorsJIASONG FANG, YONG WU, HONGGE ZHANG, YULI WEI, JUNWEI CAO, SICONG LI
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The present disclosure provides a technique for quantitatively detecting alkaline phosphatase (ALP) activities in seawater and other aquatic environments, based on surface-enhanced Raman spectroscopy by taking 5-bromo-4-chloro-3-indolyl phosphate (BCIP) as a substrate and dimethyl sulfoxide (DMSO) as an internal standard. Results show that ALP activity has a good linear correlation with the intensity ratio of a characteristic Raman peak to that of the internal standard peak (600 cm−1/677 cm−1) (R2=0.977). The technique was successfully applied to detect ALP activity of a seawater sample. By extension this technique can also be used in detecting the activity of other microbial extracellular enzymes (e.g., aminopeptidase) in seawater and thus, lays a solid scientific foundation for in-situ detection of the activities of other extracellular enzymes in seawater and other aquatic environments.
A technique for quantitatively detecting alkaline phosphatase in seawater based on surface-enhanced Raman spectroscopy, comprising the following steps:
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