Crispr cas systems and lysogeny modules
AssigneeLocus Biosciences, Inc.
InventorsDavid G. OUSTEROUT, Paul M. GAROFOLO, Kurt SELLE, Hannah Hewitt TUSON
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Abstract
Disclosed herein are compositions and methods for modifying a bacterial population. In some embodiments, described herein is a bacteriophage comprising a first nucleic acid sequence encoding a first spacer sequence or a crRNA transcribed therefrom, wherein the first spacer sequence is complementary to a target nucleotide sequence from a target gene in a target bacterium, provided that the bacteriophage is rendered lytic.
Claims78
A bacteriophage comprising a first nucleic acid sequence encoding a first spacer sequence or a crRNA transcribed therefrom, wherein the first spacer sequence is complementary to a target nucleotide sequence from a target gene in a target bacterium, provided that the bacteriophage is rendered lytic.
A temperate bacteriophage comprising a first nucleic acid sequence encoding a first spacer sequence or a crRNA transcribed therefrom, wherein the first spacer sequence is complementary to a target nucleotide sequence from a target gene in a target bacterium, provided that the bacteriophage is rendered lytic by removal of the 1247 cI repressor region.
A method for killing a target bacterium, the method comprising introducing into the target bacterium a temperate bacteriophage comprising a first nucleic acid sequence encoding a first spacer sequence or a crRNA transcribed therefrom, wherein the first spacer sequence is complementary to a target nucleotide sequence from a target gene in the target bacterium, provided that the bacteriophage is rendered lytic by a 1247 cI repressor region knockout, thereby killing the target bacterium.
Dependencies are inferred from the claim text.