System for evaluating chemo-preventive potential of phc and its prepared chitosan nanoparticles

A system for evaluating chemo-preventive potential of PHC and its prepared chitosan nanoparticles, the system comprises:

• a nanoparticle synthesis unit for synthesizing chitosan nanoparticles using polyherbal combination of ratio (2:1:1);
• a selection unit for selecting a group of rats each weighing 125-150 grams and accommodating in polypropylene cages under proper environmental conditions at room temperature of 23±2° C. and 50-60% relative humidity followed by 12 hours light/dark cycle;
• an injection for inducing Hepatocellular Carcinoma (HCC), wherein for the induction of HCC, rats administered single intraperitoneal injection of DEN (200 mg/kg body weight) followed by weekly subcutaneous injections of carbon tetrachloride (3 ml/kg body weight) for 6 weeks;
• wherein the rats are divided into eight groups, from which group 1 is served as normal control, and group 2-8 are given single dose of DEN and repeated dose of CCl4 till the completion of the experimental duration of 16 weeks;
• wherein freshly prepared solution of DEN in normal saline is used for the induction of HCC in rats by administering 200 mg/kg, i.p., PHC (2:1:1) is dispersed in normal saline suspension to administer at doses of 900 mg/kg, p.o and 1935 mg/kg p.o. Soranib (Sorafenib 200 mg) tablets are powdered and dissolve in normal saline solution and the freshly prepared solution is used as standard drug;
• a collection unit for collecting serum and tissue samples after anesthetizing overnight fasted rats using intraperitoneal administration of thiopentone sodium at a dose of 40 mg/kg, wherein the blood sample is collected by retro-orbital plexus followed by cardiac puncture;
• a treatment unit for treating the collected serum and tissue samples;
• and
• an analyzer equipped with a spectrophotometer for evaluating the chemo-preventive potential of PHC (2:1:1) and its prepared chitosan nanoparticles upon determining liver markers, antioxidant parameters, total bilirubin, protein, lipid peroxidation, and liver cancer biomarkers.