US 2022/0372516 A1 · siRNA EXPRESSION VECTOR

Abstract

Provided is a nucleic acid construct for expressing a gene of interest, the nucleic acid construct comprising, in order from 5′-terminus, each sequence of: (a) a 5′ long terminal repeat (LTR) sequence derived from a retrovirus; (b) a packaging signal sequence (IV) derived from a retrovirus; (c) a sequence or multiple cloning site of the gene of interest; (d) a post-transcriptional regulatory sequence (PRE); (e) an siRNA generating sequence which forms at least one stem-loop structure and in which RNA, which induces RNA interference in mammalian cells, is transcribed; and (f) a 3′ LTR sequence derived form a retrovirus.

Claims15

Dependent claims

A nucleic acid construct for expressing a gene of interest, comprising:

in order from the 5′ end to 3′ end,
(a) a 5′ LTR (Long Terminal Repeat) sequence derived from a retrovirus,
(b) a packaging signal sequence (w) derived from a retrovirus,
(c) a sequence of the gene of interest or a multiple cloning site,
(d) a posttranscriptional regulatory element (PRE),
(e) a siRNA-producing sequence which is transcribed into an RNA that forms at least one stem-loop structure and induces RNA interference in a mammalian cell, and
(f) a 3′ LTR sequence derived from a retrovirus.

4 more elements

Dependencies are inferred from the claim text.

siRNA EXPRESSION VECTOR

Life of this patent

siRNA EXPRESSION VECTOR

Life of this patent

Abstract

Claims15