Method for Detecting American Foulbrood in a Beehive
OwnerJulia Grassl, Jessica M. Moraninventors
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Abstract
The present invention provides a method for the detection of American foulbrood (AFB) in a beehive, said method comprising the steps of taking a sample from the beehive and testing the sample for a range of compounds, wherein the presence of one or more of the compounds in a relative amount of at least three times that of a non-infected hive indicates the presence of AFB in the beehive.
Claims8
A method for detecting the presence of American foulbrood (AFB) in a beehive, said method comprising the step of:
- a) taking a sample from the beehive;
- b) testing the sample for compounds from the list comprising:
- 1-Decene (CAS #872-05-9);
- 2(3H)-Furanone, dihydro-3-hydroxy-4,4-dimethyl-(CAS # 52126-90-6);
- 2-Coumaranone (CAS #553-86-6);
- 2-Dodecanone (CAS #6175-49-1);
- 2-Piperidinone (CAS #675-20-7);
- 3-Pentanone, 2,4-dimethyl-(CAS #565-80-0);
- 4-Hexen-3-ol (CAS #4798-58-7);
- Acetamide (CAS #60-35-5);
- C21 ketone or similar;
- Trisulfide, dimethyl-(CAS #3658-80-8);
- Disulfide, dimethyl-(CAS #624-92-0);
- Indole (CAS #120-72-9);
- N-(3-Methylbutyl)acetamide (CAS #13434-12-3);
- Pentanamide, 4-methyl-(CAS # 1119-29-5);
- Pentanoic acid, 3-methyl-2-oxo-, methyl ester (CAS #3682-42-6);
- Pyrazine, 2,5-dimethyl-(CAS #123-32-0);
- Pyrazine, trimethyl-(CAS #14667-55-1);
- Propanamide, 2-methyl-(CAS #563-83-7);
- Butanamide, 3-methyl-(CAS #541-46-8);
- Butanamide (CAS #541-35-5);
- Hexanamide (CAS #628-02-4);
- 2-Tetradecanone (CAS #2345-27-9);
- 2-Undecanone (CAS #112-12-9);
- Benzeneacetamide (CAS #103-81-1);
- Butanal, 2-methyl-(CAS # 96-17-3);
- 2-Tridecanone (CAS #593-08-8);
- 2,3-Pentanedione (CAS # 600-14-6);
- Butanal, 3-methyl-(CAS #590-86-3);
- AFB NIL 2;
- 2-Pentadecanone (CAS # 2345-28-0);
- AFB NIL 1;
- 3-Buten-1-ol, 3-methyl-(CAS #763-32-6);
- AFB NIL 6;
- AFB NIL 7;
- AFB NIL 8\ C16 alkene;
- AFB NIL 9;
- Furan, 3-methyl-(CAS #930-27-8)
- wherein the presence of one or more of the compounds in a relative amount of at least three times that of a non-infected hive indicates the presence of AFB in the beehive.
A kit for detecting the presence of American foulbrood (AFB) in a beehive, said kit comprising:
- i.) a means for testing the sample for compounds from the list comprising:
- 1-Decene (CAS #872-05-9);
- 2(3H)-Furanone, dihydro-3-hydroxy-4,4-dimethyl-(CAS #52126-90-6);
- 2-Coumaranone (CAS #553-86-6);
- 2-Dodecanone (CAS #6175-49-1);
- 2-Piperidinone (CAS #675-20-7);
- 3-Pentanone, 2,4-dimethyl-(CAS #565-80-0);
- 4-Hexen-3-ol (CAS #4798-58-7);
- Acetamide (CAS #60-35-5);
- C21 ketone or similar;
- Trisulfide, dimethyl-(CAS #3658-80-8);
- Disulfide, dimethyl-(CAS #624-92-0);
- Indole (CAS #120-72-9);
- N-(3-Methylbutyl)acetamide (CAS #13434-12-3);
- Pentanamide, 4-methyl-(CAS #1119-29-5);
- Pentanoic acid, 3-methyl-2-oxo-, methyl ester (CAS #3682-42-6);
- Pyrazine, 2,5-dimethyl-(CAS #123-32-0);
- Pyrazine, trimethyl-(CAS #14667-55-1);
- Propanamide, 2-methyl-(CAS #563-83-7);
- Butanamide, 3-methyl-(CAS #541-46-8);
- Butanamide (CAS # 541-35-5);
- Hexanamide (CAS #628-02-4);
- 2-Tetradecanone (CAS #2345-27-9);
- 2-Undecanone (CAS #112-12-9);
- Benzeneacetamide (CAS #103-81-1);
- Butanal, 2-methyl-(CAS #96-17-3);
- 2-Tridecanone (CAS #593-08-8);
- 2,3-Pentanedione (CAS #600-14-6);
- Butanal, 3-methyl-(CAS #590-86-3);
- AFB NIL 2;
- 2-Pentadecanone (CAS #2345-28-0);
- AFB NIL 1;
- 3-Buten-1-ol, 3-methyl-(CAS # 763-32-6);
- AFB NIL 6;
- AFB NIL 7;
- AFB NIL 8\ C16 alkene;
- AFB NIL 9;
- Furan, 3-methyl-(CAS #930-27-8);
- and
- ii.) instructions for use
- wherein the presence of one or more of the compounds in a relative amount of at least three times that of a non-infected hive indicates the presence of AFB in the beehive.
A device for detecting the presence of American foulbrood (AFB) in a beehive, said device comprising:
- i.) a means for testing the sample for compounds from the list comprising:
- 1-Decene (CAS #872-05-9);
- 2(3H)-Furanone, dihydro-3-hydroxy-4,4-dimethyl-(CAS # 52126-90-6);
- 2-Coumaranone (CAS #553-86-6);
- 2-Dodecanone (CAS #6175-49-1);
- 2-Piperidinone (CAS #675-20-7);
- 3-Pentanone, 2,4-dimethyl-(CAS #565-80-0);
- 4-Hexen-3-ol (CAS #4798-58-7);
- Acetamide (CAS #60-35-5);
- C21 ketone or similar;
- Trisulfide, dimethyl-(CAS #3658-80-8);
- Disulfide, dimethyl-(CAS # 624-92-0);
- Indole (CAS #120-72-9);
- N-(3-Methylbutyl)acetamide (CAS #13434-12-3);
- Pentanamide, 4-methyl-(CAS #1119-29-5);
- Pentanoic acid, 3-methyl-2-oxo-, methyl ester (CAS #3682-42-6);
- Pyrazine, 2,5-dimethyl-(CAS #123-32-0);
- Pyrazine, trimethyl-(CAS #14667-55-1);
- Propanamide, 2-methyl-(CAS # 563-83-7);
- Butanamide, 3-methyl-(CAS #541-46-8);
- Butanamide (CAS #541-35-5);
- Hexanamide (CAS #628-02-4);
- 2-Tetradecanone (CAS #2345-27-9);
- 2-Undecanone (CAS #112-12-9);
- Benzeneacetamide (CAS #103-81-1);
- Butanal, 2-methyl-(CAS #96-17-3);
- 2-Tridecanone (CAS #593-08-8);
- 2,3-Pentanedione (CAS #600-14-6);
- Butanal, 3-methyl-(CAS #590-86-3);
- AFB NIL 2;
- 2-Pentadecanone (CAS #2345-28-0);
- AFB NIL 1;
- 3-Buten-1-ol, 3-methyl-(CAS # 763-32-6);
- AFB NIL 6;
- AFB NIL 7;
- AFB NIL 8\ C16 alkene;
- AFB NIL 9;
- Furan, 3-methyl-(CAS #930-27-8)
- wherein the presence of one or more of the compounds in a relative amount of at least three times that of a non-infected hive indicates the presence of AFB in the beehive.
Dependencies are inferred from the claim text.