Engineered guanylate kinase variant enzymes
AssigneeCodexis, Inc.
InventorsDa Duan, Auric Anthony Sowell-Kantz, Aksiniya Lyubenova Petkova, Oscar Alvizo
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Abstract
The present invention provides engineered guanylate kinase (GK) enzymes, polypeptides having GK activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing GK enzymes are also provided. The present invention further provides compositions comprising the GK enzymes and methods of using the engineered GK enzymes. The present invention finds particular use in the production of pharmaceutical compounds.
Claims27
An engineered guanylate kinase comprising a polypeptide sequence having at least 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% sequence identity to SEQ ID NOs:
- 4 and/or 46, or a functional fragment thereof, wherein the polypeptide sequence of said engineered guanylate kinase comprises at least one substitution or substitution set and wherein the amino acid positions of said polypeptide sequence are numbered with reference to SEQ ID NOs:
- 4 and/or 46.
A polynucleotide sequence encoding at least one engineered guanylate kinase, said polynucleotide sequence comprising at least 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% sequence identity to SEQ ID NOs:
- 3 and/or 45, wherein the polynucleotide sequence of said engineered guanylate kinase comprises at least one substitution at one or more positions.
A polynucleotide sequence encoding at least one engineered guanylate kinase comprising at least 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% sequence identity to SEQ ID NOs:
- 3 and/or 45, or a functional fragment thereof.
Dependencies are inferred from the claim text.