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US 2022/0276267 A1Published application
Mass spectrometric quantitation assay for metabolites of leflunomide
AssigneeQuest Diagnostics Investments Incorporated
InventorKarin O. Thomassian
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Abstract
Methods are described for determining the amount of metabolites of leflunomide in a sample. More specifically, mass spectrometric methods are described for detecting and quantifying teriflunomide in a sample.
Claims10
A method for determining the amount of teriflunomide in a sample by mass spectrometry, said method comprising:
- a. adding an internal standard to the sample;
- b. purifying the sample by high turbulence liquid chromatography (HTLC);
- c. ionizing the sample to produce one or more teriflunomide ions detectable by mass spectrometry;
- d. determining the amount of the one or more teriflunomide ions by mass spectrometry;
- and
- e. using the amount of the one or more ions determined in step (d) to determine the amount of teriflunomide in the sample;
- and
- f. confirming the identity of teriflunomide by detecting a qualifier ion with m/z of 142.2±0.50.
5 more elements
Dependencies are inferred from the claim text.