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US 2022/0275407 A1Published application
Method for producing jasmonic acid by solid state fermentation of microorganisms
AssigneesWuxi New Way Biotech Co.Ltd, Jiangnan University
InventorsPu ZHENG, Ziqiang SHEN, Dan WU, Pengcheng CHEN, Ruiying LI
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Abstract
The disclosure discloses a method for producing jasmonic acid by solid state fermentation of microorganisms, and belongs to the field of biotechnology. Lasiodiplodia sp. is inoculated in a solid state fermentation medium containing wheat for static fermentation for 6-15 days, and then, jasmonic acid of 4128 mg/kg wheat can be produced. The method provides a feasibility for extension of production of jasmonic acid by fermentation of microorganisms.
Claims7
A method for producing jasmonic acid by solid state fermentation of microorganisms, comprising:
- inoculating Lasiodiplodia sp. in a solid state fermentation medium for static culture for 6-15 days at a culture temperature of 20-35° C. and an air humidity of 80-100%, where in a water content of the solid state fermentation medium is 20-80%, each kg of the solid state fermentation medium contains 200-800 g of a solid substrate, 1-15 g of sodium nitrate, 0.5-3 g of potassium dihydrogen phosphate, 0.1-0.4 g of potassium chloride, 1-9 g of magnesium salt, 0.1-0.9 g of ferric salt, 0.055-5.55 g of calcium chloride and 1-5 g of corn steep liquor, and the solid substrate is wheat bran, wheat, or a mixture of wheat bran and wheat.
Dependencies are inferred from the claim text.