Electrokinetic microelectrode devices and methods for biomarker analysis

A method of analyzing one or more biomarkers in a biofluid, comprising:

• obtaining a biofluid from a subject, wherein the biofluid is placed in a collection tube including an electrokinetic microelectrode array including:
• a support structure,
• an array of microelectrodes coupled to a first side of the support structure,
• a permeation layer at least partially formed on the array of microelectrodes over the support structure, and
• one or more detectors, sensors, and/or emitters coupled to a second side of the support structure,
• wherein the electrokinetic microelectrode array includes a folded or a sandwiched configuration of the array of microelectrodes on the first side of the support structure and the one or more detectors, sensors, emitters, or combination thereof on the second side of the support structure that forms a microfluidic channel;
• contacting the biofluid with the electrokinetic microelectrode array to separate and isolate the one or more biomarkers in the electrokinetic microelectrode array, wherein the electrokinetic microelectrode array separates and isolates the one or more biomarker(s) from the biofluid using one or both of AC dielectrophoretic (DEP) and DC electrophoretic based forces;
• and
• analyzing the one or more biomarkers in the biofluid.