Methods and products for treatment of gastrointestinal disorders

A method of preparing a therapeutic bacterial isolate, the method comprising:

• (i) providing a preparation of bacteria from fecal material from
• (a) a group of healthy subjects,
• (b) a group of patients with a gastrointestinal disease or disorder, and
• (c) a group of patients having clinical remission of one or more symptoms of the gastrointestinal disease or disorder following treatment of each patient of the group of patients with a fecal microbiota transplant,
• (ii) analyzing 16S rRNA sequences from the preparation of bacteria, and
• (iii) isolating a bacterial isolate from stool of a human donor that comprises a 16S rRNA sequence that is at least 97% identical to a 16S rRNA sequence of a bacterial strain from the preparation of bacteria that is
• (a) enriched in a group of healthy subjects over a group of patients with the gastrointestinal disease or disorder;
• and/or
• (b) correlated with clinical remission of one or more symptoms of the gastrointestinal disease or disorder in a group of patients following treatment of each patient of the group of patients with the fecal microbiota transplant,
• wherein a cross-sectional combined p-value of the bacterial isolate is less than 1×10−14.