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US 2022/0220529 A1Published application
Method of screening inhibitor of caspase activity by lipopolysaccharide
AssigneeKorea Institute of Science and Technology
InventorsHak Suk CHUNG, Jinsu AN, Eun Gyeong YANG, So Yeon KIM
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Abstract
Provided are a method of screening an inhibitor of caspase activity by lipopolysaccharide and a method of screening a therapeutic agent for inflammatory diseases or sepsis using the same. Accordingly, it is possible to develop a caspase-4-specific inhibitor.
Claims14
A method of screening an inhibitor of caspase activity by lipopolysaccharide (LPS), the method comprising:
- incubating a mixture comprising a polypeptide, lipopolysaccharide (LPS), and a test material, the polypeptide comprising caspase activation and recruitment domain (CARD) labeled with a fluorescent material;
- measuring a fluorescence polarization (FP) value of the mixture;
- and
- selecting the test material as an inhibitor of binding of CARD and LPS, when the measured FP value is lowered, as compared with that of a negative control not treated with the test material.
1 more element
A method of screening a therapeutic agent for inflammatory diseases or sepsis, the method comprising:
- incubating a mixture comprising a polypeptide, lipopolysaccharide (LPS), and a test material, the polypeptide comprising caspase activation and recruitment domain (CARD) labeled with a fluorescent material;
- measuring a fluorescence polarization (FP) value of the mixture;
- and
- selecting the test material as a therapeutic agent candidate for inflammatory diseases or sepsis, when the measured FP value is lowered, as compared with that of a negative control not treated with the test material.
1 more element
Dependencies are inferred from the claim text.