US 2022/0213526 A1Published application

Methods of Detecting Analytes

AssigneeSpatial Transcriptomics AB

InventorsJonas Frisen, Patrik Stahl, Joakim Lundeberg

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Abstract

Localized detection of RNA in a tissue sample that includes cells is accomplished on an array. The array include a number of features on a substrate. Each feature includes a different capture probe immobilized such that the capture probe has a free 3′ end. Each feature occupies a distinct position on the array and has an area of less than about 1 mm2. Each capture probe is a nucleic acid molecule, which includes a positional domain including a nucleotide sequence unique to a particular feature, and a capture domain including a nucleotide sequence complementary to the RNA to be detected. The capture domain can be at a position 3′ of the positional domain.

Claims1

Dependent claims

A method for spatial detection of a nucleic acid in a tissue sample comprising:

(a) providing an array comprising a plurality of capture probes, wherein a capture probe of the plurality of capture probes comprises:
(i) a random nucleic acid sequence, and
(ii) a capture domain;
(b) contacting the tissue sample with the array and allowing the nucleic acid of the tissue sample to interact with the capture domain of the capture probe;
(c) generating DNA molecules from the captured nucleic acid;
(d) analyzing the generated DNA molecules, thereby determining the random nucleic acid sequence that is associated with the captured nucleic acid;
and
(e) correlating the determined random nucleic acid sequence to a position on the array.

5 more elements

Dependencies are inferred from the claim text.

Facts

Application: 17/704,830Filed 25 Mar 2022
Priority: GB 1106254.413 Apr 2011
Published: 7 Jul 2022
Term ends: Not estimatedThe source records don’t support an estimate.

Classification21 codes

C12Q 1/6837
Enzymatic or biochemical coupling of nucleic acids to a solid phase: using probe arrays or probe chips
C12N 15/1065
Isolating an individual clone by screening libraries: preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis,…
C12Q 1/6806
Involving nucleic acids: preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
C12Q 1/6816
Hybridisation assays: characterised by the detection means

Family

Application14/111,482Continuation · filed 11 Oct 2013
Application16/013,654Continuation · filed 20 Jun 2018
Application17/474,922Continuation · filed 14 Sep 2021
Application17/704,830Continuation
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US 2014/0066318 A1Published application
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US 2022/0127659 A1Published application
US 2022/0213526 A1This patent

From the US filing history. Foreign family members aren’t listed.

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