Method of diagnosis
AssigneeThe University of Liverpool
InventorsEithne Costello, William Greenhalf, Lucy Oldfield, Christopher Halloran, Paula Ghaneh
Life of this patent
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Abstract
The present disclosure relates to a diagnostic test for the early detection of pancreatic cancer [i.e. pancreatic ductal adenocarcinoma, ‘PDAC’] or pancreatitis in a cohort of patients selected for an increased risk of PDAC which is associated with diabetes mellitus (DM) and including treatment regimens for the treatment of subjects with PDAC and kits used in the method of the invention.
Claims25
An immunoassay to determine whether a subject has elevated levels of an adiponectin polypeptide and an IL-1Ra polypeptide comprising or consisting of the steps:
- i) obtaining a biological sample from a subject to be tested;
- ii) forming a preparation comprising said sample and an antibody or antibodies that bind adiponectin and an antibody or antibodies that bind IL-1Ra to form an antibody/adiponectin polypeptide complex and an antibody/IL-1Ra polypeptide complex;
- iii) detecting each complex;
- and
- iv) comparing the level of adiponectin and IL-1Ra to a relevant matched control.
An immunoassay method to determine whether a subject is suspected of having early stage pancreatic ductal adenocarcinoma or pancreatitis comprising or consisting of the steps:
- i) obtaining a biological sample from a subject to be tested;
- ii) forming a preparation comprising said sample and an antibody or antibodies that bind an adiponectin and an antibody or antibodies that bind IL-1Ra to form an antibody/adiponectin polypeptide complex and an antibody/IL-1Ra polypeptide complex;
- iii) detecting each complex;
- and
- iv) comparing the level of adiponectin polypeptide and IL-1Ra polypeptide to a relevant matched control.
(canceled)
(canceled)
11-12. (canceled)
A solid support comprising immobilised antibodies that specifically bind adiponectin and/or IL-1Ra.
A kit comprising or consisting of:
- an antibody that specifically binds an adiponectin polypeptide;
- an antibody that specifically binds an IL-1Ra polypeptide;
- and
- secondary antibodies that bind said monoclonal antibody that binds an adiponectin polypeptide and secondary antibodies that bind said monoclonal antibody that binds an IL-1Ra polypeptide wherein said secondary antibodies comprise separate detectable labels.
26-27. (canceled)
Dependencies are inferred from the claim text.