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AssigneeIntegrated Micro-Chromatography Systems, Inc.
InventorsJohn TOMASHEK, Caleb Reece SCHLACHTER, Pongkwan SITASUWAN, Lim Andrew LEE
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Blends of enzymes of the same enzyme class (same EC number), such as beta-glucuronidase enzyme blends, are provided that exhibit synergistic levels of activity across a range of substrates as compared to the activity levels of each enzyme in the blend individually. The blends also may exhibit a greater effective substrate range, as well as greater effective pH and/or temperature ranges as compared to single enzyme preparations. Predictive methods for determining optimal enzyme blends, methods for preparing enzyme blends, enzyme blend compositions, enzyme blend formulations and methods of using such enzyme blends are provided.
A composition comprising a blend of two or more enzymes, wherein each enzyme in the blend has the same Enzyme Commission (EC) number and wherein the level of activity of the blend for a range of substrates catalyzed by the blend is synergistically greater than the level of activity for the range of substrates catalyzed by each enzyme in the blend individually.
A composition comprising a blend of two or more beta-glucuronidase enzymes (Enzyme Commission number 3.2.1.31), wherein:
A composition comprising a blend of Eubacterium eligens beta-glucuronidase (EeGUS) and a chimeric beta-glucuronidase enzyme, Rxn3, comprising the amino acid sequence shown in SEQ ID NO:
A method of preparing an enzyme blend that is optimized for a given temperature, pH, and substrate target range, wherein each enzyme within the blend has the same EC number and wherein the specific activity of each enzyme on each substrate for the given temperature and pH is known, the method comprising:
Dependencies are inferred from the claim text.