ACYL-ACP Reductase With Improved Properties
AssigneeGenomatica, Inc.
InventorsMathew RUDE, Na TRINH, Andreas SCHIRMER, Jacob GANO
Life of this patent
Estimating the term…
Abstract
The disclosure relates to acyl-ACP reductase (AAR) enzyme variants that result in improved fatty aldehyde and fatty alcohol production when expressed in recombinant host cells. The disclosure further relates to methods of making and using such AAR variants for the production of fatty alcohol compositions having particular characteristics.
Claims14
-82. (canceled)
A recombinant host cell expressing a variant acyl-ACP reductase (AAR) polypeptide comprising at least 90% sequence identity to the amino acid sequence of SEQ ID NO:
- 80, wherein said variant AAR polypeptide comprises a mutation at an amino acid position selected from the group consisting of Q40V, G52V, G273E, K303G, H340P, L344A, L344D, L344S, L344T, A345R, V346P, V346G, and A345*, and wherein said AAR polypeptide catalyzes the conversion of an acyl-ACP to a fatty aldehyde.
A cell culture comprising a recombinant host cell that expresses a variant acyl-ACP reductase (AAR) polypeptide comprising at least 90% sequence identity to the amino acid sequence of SEQ ID NO:
- 80, wherein said variant AAR polypeptide comprises a mutation at an amino acid position selected from the group consisting of Q40V, G52V, G273E, K303G, H340P, L344A, L344D, L344S, L344T, A345R, V346P, V346G, and A345*, and wherein said AAR polypeptide catalyzes the conversion of an acyl-ACP to a fatty aldehyde.
A method of producing a fatty alcohol composition having an increase in titer, comprising:
- i. culturing with a carbon source, a recombinant host cell that expresses a variant acyl-ACP reductase (AAR) polypeptide comprising at least 90% sequence identity to the amino acid sequence of SEQ ID NO:
- 80, wherein said variant AAR polypeptide comprises a mutation at an amino acid position selected from the group consisting of Q40V, G52V, G273E, K303G, H340P, L344A, L344D, L344S, L344T, A345R, V346P, V346G, and A345*, and wherein said AAR polypeptide catalyzes the conversion of an acyl-ACP to a fatty aldehyde, and
- ii. harvesting a fatty alcohol composition.
Dependencies are inferred from the claim text.