Supplemented Serum-Containing Culture Medium for Enhanced Arpe-19 Growth and Human Cytomegalovirus Vaccine Production
AssigneeMerck Sharp & Dohme Corp.
InventorsSianny Christanti, Esther Wei Yee Lim, Jason Rodriguez, Christopher L. Daniels
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Abstract
The present invention relates to supplemented serum-containing cell culture media that provides enhances ARPE-19 cell growth and/or improves the yield of human cytomegalovirus (HCMV) grown in ARPE-19 cell cultures. The media of the invention includes two additives, a hormone (e.g., a glucocorticoid hormone such as dexamethasone) and a growth factor (e.g., EGF). The invention further provides methods of producing HCMV in such growth media.
Claims20
A cell culture medium comprising:
- a basal cell culture medium comprising;
- a serum comprising between about 0.1 μM and about 10 μM of dexamethasone, and between about 0.1 ng/mL and about 100 ng/mL of epidermal growth factor (EGF).
A method of increasing virus production in cells comprising culturing a cell culture medium comprising:
- a basal cell culture medium comprising:
- a serum comprising dexamethasone in the amount of about 0.1 μM to about 10 μM;
- epidermal growth factor (EGF) in the amount of about 0.1 ng/mL to about 100 ng/mL;
- wherein the composition provides increased production of the virus by at least 10% relative to the production of virus grown in a composition that does not include the basal cell culture.
Dependencies are inferred from the claim text.