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US 2022/0089785 A1Published application
Compositions and methods for t-cell receptor gene assembly
AssigneeRootPath Genomics, Inc.
InventorsXi Chen, Ely Porter
Life of this patent
Estimating the term…
Abstract
Provided herein are compositions and methods for assembling nucleic acid sequences encoding T-cell receptors.
Claims21
-58. (canceled)
A composition comprising
- (a) a plurality of nucleic acid molecules, wherein each nucleic acid molecule of the plurality of nucleic acid molecules comprises a sequence derived from a T-cell receptor (TCR) V gene and does not comprise a complementarity determining region 3 (CDR3) sequence, wherein a first nucleic acid molecule of the plurality comprises a first anti-connector sequence and a second nucleic acid molecule of the plurality comprises a second anti-connector sequence, wherein the first anti-connector sequence is different from the second anti-connector sequence, and wherein the sequence derived from a TCR V gene of the first nucleic acid molecule and the second nucleic acid molecule are derived from a different TCR V gene;
- and
- (b) at least one nucleic acid molecule comprising a sequence encoding a CDR3 of a TCR chain, wherein the at least one nucleic acid molecule further comprises a first connector sequence complementary to the first anti-connector sequence such that the at least one nucleic acid molecule is capable of specifically linking to the first nucleic acid molecule of (a) comprising the first anti-connector sequence.
Dependencies are inferred from the claim text.