Antimicrobial peptides
AssigneeDonald Danforth Plant Science Center
InventorsHui Li, Dilip M. Shah, Siva L.S. Velivelli
Life of this patent
Estimating the term…
Abstract
Antimicrobial OeDef1-, MtDef6-, or OeDef7-type peptides and proteins are disclosed along with compositions comprising the OeDef1-, MtDef6-, or OeDef7-type peptides and proteins and transgenic or genetically edited plants or microorganisms that express the OeDef1-, MtDef6-, or OeDef7-type peptides and proteins to inhibit growth of pathogenic microbes. Such OeDef1-, Mt-Def6-, or OeDef7-type peptides and proteins, compositions, plants, and microorganisms can provide for inhibition of microbial growth.
Claims104
A recombinant polynucleotide comprising a polynucleotide encoding a first antimicrobial peptide comprising:
- (i) an amino acid sequence having at least 60%, 70%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, 99%, or 100% sequence identity across the entire length of SEQ ID NO:
- 1, SEQ ID NO:
- 35, SEQ ID NO:
- 36, or SEQ ID NO:
- 38;
- (ii) an amino acid sequence of SEQ ID NO:
- 33 or a variant thereof wherein one or more of the hydrophobic, basic, and/or acidic amino acid residues are substituted with hydrophobic, basic, and/or acidic amino acid residues, respectively;
- (iii) an amino acid sequence having at least 90%, 92%, 95%, 97%, 98%, 99%, or 100% sequence identity to SEQ ID NO:47;
- or (iv) an amino acid sequence having at least 80%, 85%, 90%, 92%, 95%, 97%, 98%, 99%, or 100% sequence identity to SEQ ID NO:
- 54;
- wherein the first antimicrobial peptide comprises a defensin gamma core peptide, and wherein the polynucleotide encoding the first antimicrobial peptide is operably linked to a polynucleotide comprising a promoter which is heterologous to the polynucleotide encoding the first antimicrobial peptide.
An edited polynucleotide comprising a variant polynucleotide encoding a first antimicrobial peptide comprising:
- (i) an amino acid sequence having at least 60%, 70%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, 99%, or 100% sequence identity across the entire length of SEQ ID NO:
- 1, SEQ ID NO:
- 35, SEQ ID NO:
- 36, or SEQ ID NO:
- 38;
- (ii) an amino acid sequence of SEQ ID NO:
- 33 or a variant thereof wherein one or more of the hydrophobic, basic, and/or acidic amino acid residues are substituted with hydrophobic, basic, and/or acidic amino acid residues, respectively;
- (iii) an amino acid sequence having at least 90%, 92%, 95%, 97%, 98%, 99%, or 100% sequence identity to SEQ ID NO:47;
- or (iv) an amino acid sequence having at least 80%, 85%, 90%, 92%, 95%, 97%, 98%, 99%, or 100% sequence identity to SEQ ID NO:
- 54;
- wherein the first antimicrobial peptide comprises a defensin gamma core peptide, wherein the variant polynucleotide is operably linked to a polynucleotide comprising a promoter, wherein the variant polynucleotide sequence comprises at least one nucleotide insertion, deletion, and/or substitution in comparison to the corresponding wild type polynucleotide sequence, and wherein the corresponding unedited wild type polynucleotide sequence does not encode the antimicrobial peptide comprising the amino acid sequence of SEQ ID NO:
- 1 or 54.
A plant nuclear or plastid genome comprising a polynucleotide encoding a first antimicrobial peptide comprising:
- (i) an amino acid sequence having at least 60%, 70%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, 99%, or 100% sequence identity across the entire length of SEQ ID NO:
- 1, SEQ ID NO:
- 35, SEQ ID NO:
- 36, or SEQ ID NO:
- 38;
- (ii) an amino acid sequence of SEQ ID NO:
- 33 or a variant thereof wherein one or more of the hydrophobic, basic, and/or acidic amino acid residues are substituted with hydrophobic, basic, and/or acidic amino acid residues, respectively;
- (iii) an amino acid sequence having at least 90%, 92%, 95%, 97%, 98%, 99%, or 100% sequence identity to SEQ ID NO:47;
- or (iv) an amino acid sequence having at least 80%, 85%, 90%, 92%, 95%, 97%, 98%, 99%, or 100% sequence identity to SEQ ID NO:
- 54;
- wherein the first antimicrobial peptide comprises a defensin gamma core peptide, and wherein the polynucleotide is heterologous to the nuclear or plastid genome and wherein the polynucleotide is operably linked to an endogenous promoter of the nuclear or plastid genome.
A composition comprising a first antimicrobial peptide comprising:
- (i) an amino acid sequence having at least 60%, 70%, 80%, 85%, 90%, 92%, 95%, 97%, 98%, 99%, or 100% sequence identity across the entire length of SEQ ID NO:
- 1, SEQ ID NO:
- 35, SEQ ID NO:
- 36, or SEQ ID NO:
- 38;
- (ii) an amino acid sequence of SEQ ID NO:
- 33 or a variant thereof wherein one or more of the hydrophobic, basic, and/or acidic amino acid residues are substituted with hydrophobic, basic, and/or acidic amino acid residues, respectively;
- (iii) an amino acid sequence having at least 90%, 92%, 95%, 97%, 98%, 99%, or 100% sequence identity to SEQ ID NO:47;
- or (iv) an amino acid sequence having at least 80%, 85%, 90%, 92%, 95%, 97%, 98%, 99%, or 100% sequence identity to SEQ ID NO:
- 54;
- wherein the first antimicrobial peptide comprises a defensin gamma core peptide, said composition further comprising an agriculturally, pharmaceutically, or veterinarily acceptable carrier, diluent, or excipient.
Dependencies are inferred from the claim text.