Method for preparing gos-preparation with beta-galactosidase from cryptococcus terrestris, gos preparations obtainable thereby and uses thereof

2:

• The method according to claim 1, wherein the lactose feed comprises a demineralized CWP, preferably CWP that has been demineralized, more preferably by electrodialysis (ED), to an ash content of up to 4 wt. % and/or a conductivity of up to 4 mS.

3:

• The method according to claim 1, wherein the lactose feed is contacted with beta-galactosidase at a temperature of about 65-70° C., at a lactose content in the range of 47-55 wt. % lactose, and/or an enzyme dosage of 0.75-1.0 LU/gram.

4:

• The method according to claim 1, wherein the lactose feed comprises a SL-CWP, preferably having sialyllactose content of at least 0.25 wt. %, more preferably at least 0.3 wt. % based on dry solids.

5:

• The method according to claim 4, wherein the SL-CWP is a CWP that has been subjected to a lactose-removal step, preferably followed by a step to remove multivalent anions.

6:

• The method according to claim 4, wherein the lactose-content of the SL-CWP is about 52-60 wt. %.

7:

• The method according to claim 4, wherein the lactose feed is contacted with beta-galactosidase at a temperature of about 55-60° C., at pH 6.0-7.0 and/or at an enzyme dosage of 0.75-1.0 LU/gram.

8:

• The method according to claim 1, wherein the HA-GOS thus obtained is subjected to at least one further purification step, preferably wherein said purification step comprises one or more of demineralization, de-proteinization, removal of mono-sugar components, enriching for sialyllactose and removal of coloured impurities.

9:

• The method according to claim 1, wherein the beta-galactosidase comprises an amino acid sequence that is at least 85%, preferably at least 90%, more preferably at least 95%, yet even more preferably at least 97%, and most preferably at least 98% identical to any of SEQ ID NO:
• 1, 2, 3 or 4.

10:

• The method according to claim 1, wherein the beta-galactosidase (EC 3.2.1.23) is derived from Cryptococcus terrestris strain MM13-F2171 (Accession Number:
• NITE BP-02177) or APC-6431 (Accession Number:
• NITE BP-02178).

11:

• A hypoallergenic GOS preparation obtained by the method according to claim 1.

12:

• The hypoallergenic GOS preparation according to claim 11, showing a decreased score in a Basophil Activation Test performed on a blood sample isolated from a subject when compared to a GOS preparation obtained by transgalactosylation of lactose using Bacillus circulans beta-galactosidase.

13:

• A method of at least partially preventing an (IgE-mediated) allergic response in a subject known to suffer or has an increased chance to suffer from hypersensitivity to a GOS preparation obtained by transgalactosylation of lactose using a beta-galactosidase derived from Bacillus circulans, the method comprising administering to the subject an effective amount of the hypoallergenic GOS preparation of claim 11.

14:

• A nutritional composition comprising the hypoallergenic GOS preparation according to claim 11.

15:

• The nutritional composition according to claim 14, wherein the nutritional composition is a MUM composition for pregnant women, a growing up milk (GUM), a follow-up formula, or an infant formula.