Base mutation detection method and apparatus based on sequencing data, and storage medium

A base mutation detection apparatus based on sequencing data, the base mutation detection apparatus comprising:

• an initial frequency determination module configured to determine an initial frequency of an initial frequency of sequencing data of a plurality of samples to be detected being a specific base at an interested locus;
• an expected value calculation module configured to calculate, based on the initial frequency, an expected value of each of the plurality of samples to be detected being the specific base at the interested locus;
• an update module configured to update, by using each expected value, the initial frequency of the sequencing data of the plurality of samples to be detected being the specific base at the interested locus;
• an iteration module configured to:
• further calculate, by using the updated initial frequency, the expected value of each of the plurality of samples to be detected being the specific base at the interested locus, further update, by using each new expected value, the initial frequency of the sequencing data of the plurality of samples to be detected being the specific base at the interested locus, and repeat the foregoing iteration until the expected value of each of the plurality of samples to be detected being the specific base at the interested locus converges;
• and
• a mutation type determination module configured to determine, based on each converging expected value, a base mutation type and a mutation confidence at the interested locus of each of the plurality of samples to be detected,
• wherein the specific base comprises an adenine A base, a thymine T base, a cytosine C base, or a guanine G base.