Equalizer-based intensity correction for base calling

A non-transitory computer readable storage medium impressed with computer program instructions to perform base calling, the instructions, when executed on a processor, implement a method comprising:

• accessing an image whose pixels depict intensity emissions from a target cluster and intensity emissions from additional adjacent clusters;
• selecting a lookup table that contains pixel coefficients that are configured to increase a signal-to-noise ratio;
• applying the pixel coefficients to intensity values of the pixels in the image to produce an output;
• and
• base calling the target cluster based on the output.

A system including one or more processors coupled to memory, the memory loaded with computer instructions to perform base calling, the instructions, when executed on the processors, implement actions comprising:

• accessing an image whose pixels depict intensity emissions from a target cluster and intensity emissions from additional adjacent clusters;
• selecting a lookup table that contains pixel coefficients that are configured to increase a signal-to-noise ratio;
• applying the pixel coefficients to intensity values of the pixels in the image to produce an output;
• and
• base calling the target cluster based on the output.

A computer-implemented method of base calling, the method including:

• accessing an image whose pixels depict intensity emissions from a target cluster and intensity emissions from additional adjacent clusters;
• selecting a lookup table that contains pixel coefficients that are configured to increase a signal-to-noise ratio;
• convolving the pixel coefficients with intensity values of the pixels in the image to produce convolved features;
• interpolating the convolved features to generate an output;
• and
• base calling the target cluster based on the output.