Flat-Field Imaging System and Methods of Use

A method for analyzing targets comprising biological sample, the method comprising:

• forming a volume, wherein the volume comprises a third phase in fluid communication with a first phase and a second phase and is within a field of view of an optical sensor,
• wherein the third phase includes a plurality of targets comprising biological sample, and
• wherein the plurality of targets are disposed in a substantially non-overlapping single layer within the volume;
• amplifying the biological sample;
• and
• imaging, using the optical sensor, the plurality of targets, to determine amplification of the biological sample.

A system for analyzing targets comprising biological sample, the system comprising:

• a volume, wherein the volume comprises a third phase in fluid communication with a first phase and a second phase,
• wherein the third phase includes a plurality of targets comprising biological sample, and
• wherein the plurality of targets are disposed in a substantially non-overlapping single layer within the volume;
• a thermo cycler for amplifying the biological sample;
• and
• an optical sensor having a field of view including the third phase for imaging the plurality of targets in the volume.