Phospholipase C Mutant With High Enzyme Activity
AssigneeWilmar (Shanghai) Biotechnology Research & Development Center Co., Ltd.
InventorsYaoji XUAN, Qiwen NIU, Zhengjun XU
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Abstract
Provided in the present invention is a phospholipase C mutant with high enzyme activity, a polypeptide with the sequence as shown in SEQ ID NO:7, or a polypeptide derived from the phospholipase C formed by performing substitution, deletion or addition of one or a plurality of amino acids on the polypeptide of SEQ ID NO:7 while retaining the phospholipase C activity provided by SEQ ID NO:7. The phospholipase C mutant of the present invention can improve degumming efficiency and increase the yield of diacylglycerol (DAG) during degumming.
Claims21
An isolated polypeptide, selected from the group consisting of:
- (1) a polypeptide shown by SEQ ID NO:
- 7;
- (2) a polypeptide having at least 80% sequence identity to SEQ ID NO:7, wherein the amino acid residues of the polypeptide that correspond to at least one of amino acids 6, 8, 10 and 104 of SEQ ID NO:
- 7 are identical to amino acids 6, 8, 10 and/or 104 of SEQ ID NO:
- 7, respectively, and the polypeptide has phospholipase C activity of SEQ ID NO:
- 7;
- and
- (3) a polypeptide having at least 80% sequence identity to SEQ ID NO:4, wherein the amino acid residues of the isolated polypeptide that correspond to amino acids 6, 8, 10 and 104 of SEQ ID NO:
- 4 are proline, valine, serine and serine, respectively.
(canceled)
Dependencies are inferred from the claim text.