Alignment of cells in engineered tissues
AssigneeRegents of the University of Minnesota
InventorsCaleb Darwin Vogt, Angela Panoskaltsis-Mortari
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Abstract
Devices, systems, and techniques are described for printing pre-aligned microtissues into larger tissue constructs. For example, a method of printing a tissue construct includes aligning cells in a first direction to create pre-aligned microtissues, suspending the pre-aligned microtissues in a liquid to create a bioink, and depositing the pre-aligned microtissues in a second direction to create the tissue construct.
Claims20
A method of printing a tissue construct, the method comprising:
- aligning cells in a first direction to create pre-aligned microtissues;
- suspending the pre-aligned microtissues in a liquid to create a bioink;
- and
- depositing the pre-aligned microtissues in a second direction to create the tissue construct.
A 3D-printed tissue construct comprising:
- a plurality of pre-aligned microtissues, each pre-aligned microtissues comprising cells aligned in a first direction, wherein the plurality of pre-aligned microtissues deposited via a bioink in a second direction to form the 3D-printed tissue construct.
A system for printing a tissue construct, the system comprising:
- a first bioreactor configured to mature and align cells in a first direction to create pre-aligned microtissues;
- a printer nozzle configured to deposit the pre-aligned microtissues in a second direction to create the tissue construct;
- and
- a second bioreactor to mature the tissue construct.
Dependencies are inferred from the claim text.