Recombinant polyclonal proteins targeting zika and methods of use thereof

An isolated protein comprising a cognate pair of heavy chain and light chain, wherein

• a. a heavy chain CDR1 having the sequence of GFTFSSYA (SEQ ID NO:
• 25841), a heavy chain CDR2 having the sequence of ISGSGGST (SEQ ID NO:
• 25847), and a heavy chain CDR3 having the sequence of AKEGMVLRYFDWLWDY (SEQ ID NO:
• 25856);
• b. a heavy chain CDR1 having the sequence of GFTFSSYG (SEQ ID NO:
• 25842), a heavy chain CDR2 having the sequence of I[W/S]YDGSNK (SEQ ID NO:
• 25848), and a heavy chain CDR3 having the sequence of R[D/E][R/L][S/V/L]SG[P/W]S[G/H]FDY (SEQ ID NO:
• 25857);
• c. a heavy chain CDR1 having the sequence of GFTFSSYA (SEQ ID NO:
• 25841), a heavy chain CDR2 having the sequence of ISGSGGST (SEQ ID NO:
• 25847), and a heavy chain CDR3 having the sequence of A[K/E/R][E/T][Q/L/T][L/W/I][L/V/W/N][G/L]V[V/P/L][F/L/N][D/N]Y (SEQ ID NO:
• 25858);
• d. a heavy chain CDR1 having the sequence of GFTFDD[Y/H]G (SEQ ID NO:
• 25843), a heavy chain CDR2 having the sequence of INWNG[G/D]ST (SEQ ID NO:
• 25849), and a heavy chain CDR3 having the sequence of AR[D/N][R/G]GS[S/T][G/S/W/F][W/F/Y][R/F/Y]D[W/A]FD[P/I] (SEQ ID NO:
• 25859);
• or
• e. a heavy chain CDR1 having the sequence of GFTFSD[Y/D][Y/F] (SEQ ID NO:
• 25844), a heavy chain CDR2 having the sequence of ISGSG[S/Y]TI (SEQ ID NO:
• 25850), and a heavy chain CDR3 having the sequence of ARDEGY[R/S]GY[V/N]LD[T/N/S] (SEQ ID NO:
• 25860).

A method of generating a recombinant polyclonal protein composition (RPP), comprising

• a. providing B cells, plasma cells, or plasmablasts of a donor exposed to an antigen of Zika or Dengue virus;
• b. amplifying a cognate pair of polynucleotides encoding a light chain variable region and a heavy chain variable region from each of a plurality of the plasma cells or plasmablasts by overlap extension reverse transcriptase polymerase chain reaction (OE-RT-PCR);
• c. cloning polynucleotides obtained from the amplification into expression vectors, thereby obtaining constructs encoding the RPP;
• d. expressing the RPP from the constructs;
• e. enriching a subset of the constructs based on activity of the RPP or a member of the RPP against Zika;
• and
• f. obtaining the RPP (also called a Recombinant Zika Immune Globulin (rZIG)).