Devices and methods for synthesis
AssigneeTwist Bioscience Corporation
InventorsAndres FERNANDEZ, Eugene P. Marsh, Maryam HABIBIAN
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Abstract
Provided herein are compositions, devices, systems and methods for electrochemical synthesis. Further provided are devices comprising addressable electrodes controlling polynucleotide synthesis (deprotection, extension, or cleavage, etc.) The compositions, devices, systems and methods described herein provide improved synthesis, storage, density, and retrieval of biomolecule-based information.
Claims35
A method for synthesizing a polynucleotide comprising:
- (a) contacting at least one nucleoside attached to a solid support with a protected nucleoside, wherein the protected nucleoside is configured to form a covalent bond with the at least one nucleoside to generate a protected polynucleotide;
- (b) applying a voltage to a solvent in fluid communication with the protected polynucleotide, wherein the voltage results in deprotection of a terminal nucleoside of the protected polynucleotide, and wherein the voltage is delivered as at least 2 pulses;
- (c) repeating steps (a) and (b) to synthesize the polynucleotide.
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A method for synthesizing a polynucleotide comprising:
- (a) providing a surface having (i) one or more electrodes proximal to the surface and (ii) one or more in-plane cathodes proximal to the surface, wherein the surface comprises a first plurality of protected polynucleotides attached thereto;
- (b) energizing at least one electrode proximal to a first region of the surface to electrochemically generate a deprotection reagent, wherein the deprotection reagent deprotects at least some of the first plurality of polynucleotides in the first region;
- (c) coupling at least one protected nucleoside to at least one deprotected polynucleotide in the first region;
- and
- (d) repeating steps (a)-(c) to synthesize the polynucleotide.
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A device comprising at least one addressable solid support, wherein the at least one solid support comprises:
- a base layer comprising silicon;
- an intermediate layer comprising a conductive material, wherein the intermediate layer is configured to produce an electrochemically generated reagent when energized with a voltage;
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- a top layer comprising an oxide, wherein the intermediate layer and the top layer are in fluid communication with a solvent, and wherein the intermediate layer is located between the base layer and the top layer, and the top layer is configured for the attachment of molecules, and
- wherein the solid support comprises a plurality of features.
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Dependencies are inferred from the claim text.