Computational systems pathology spatial analysis platform for in situ or in vitro multi-parameter cellular and subcellular imaging data

A computerized system for analyzing a progression of a disease from multi-parameter cellular and subcellular imaging data obtained from a plurality of tissue samples from a plurality of patients or a number of multicellular in vitro models, comprising:

• a processing apparatus, wherein the processing apparatus includes:
• a spatial heterogeneity quantification component configured for generating a global quantification of a spatial heterogeneity among cells of certain varying predetermined phenotypes in the multi-parameter cellular and subcellular imaging data;
• a microdomain identification component configured for identifying a plurality of microdomains for a plurality of tissue sections from the multi-parameter cellular and subcellular imaging data based on the global quantification, each microdomain being associated with a respective one of the tissue sections;
• and
• a weighted graph component configured for constructing a weighted graph for the multi-parameter cellular and subcellular imaging data, the weighted graph being indicative of a progression of the predetermined phenotypes and having a plurality of nodes and a plurality of edges each being located between a pair of the nodes, wherein in the weighted graph each node is a particular one of the microdomains and the edge between each pair of microdomains in the weighted graph is indicative of a degree of similarity between the pair of the microdomains, such that the weighted graph may be analyzed to understand the progression of the disease.