System and method for on-phase microscopy
AssigneeThe Joan and Irwin Jacobs Technion-Cornell Institute
InventorShahar BARBASH
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Abstract
Techniques for processing images to be used for more accurate measurement of biological processes such as cell migration, as well as techniques for measuring cell migration. A method for processing microscopic images includes generating a smoothed image for a raw image by applying a smoothing filter to the raw image, wherein the raw image shows a plurality of cells and a background; generating a high pass filter image by dividing the raw image by the smoothed image; and transforming the high pass filter image into a transformed image by augmenting the spatial frequency of the plurality of cells shown in the high pass filter image with respect to the background.
Claims20
A method for processing microscopic images, comprising:
- generating a smoothed image for a raw image by applying a smoothing filter to the raw image, wherein the raw image shows a plurality of cells and a background;
- generating a high pass filter image by dividing the raw image by the smoothed image;
- and
- transforming the high pass filter image into a transformed image by augmenting the spatial frequency of the plurality of cells shown in the high pass filter image with respect to the background.
A non-transitory computer readable medium having stored thereon instructions for causing a processing circuitry to execute a process, the process comprising:
- generating a smoothed image for a raw image by applying a smoothing filter to the raw image, wherein the raw image shows a plurality of cells and a background;
- generating a high pass filter image by dividing the raw image by the smoothed image;
- and
- transforming the high pass filter image into a transformed image by augmenting the spatial frequency of the plurality of cells shown in the high pass filter image with respect to the background.
A system for processing images, comprising:
- a processing circuitry;
- and
- a memory, the memory containing instructions that, when executed by the processing circuitry, configure the system to:
- generate a smoothed image for a raw image by applying a smoothing filter to the raw image, wherein the raw image shows a plurality of cells and a background;
- generate a high pass filter image by dividing the raw image by the smoothed image;
- and
- transform the high pass filter image into a transformed image by augmenting the spatial frequency of the plurality of cells shown in the high pass filter image with respect to the background.
A method for processing microscopic images, comprising:
- selecting a sensitivity by iteratively applying a threshold to an image until a sum of cells shown in the image is steady, wherein the threshold is applied at each iteration using a current sensitivity, wherein the sum of cells in the image is determined to be steady when the sum of cells remains within a threshold of an initial sum of cells after a predetermined period of time, wherein the selected sensitivity is the current sensitivity being used when the sum of cells shown in the image is determined to be steady;
- and
- generating a processed image using the selected sensitivity.
Dependencies are inferred from the claim text.