Microscopic transmitted light contrasting method

A device for carrying out a microscopic transmitted light contrasting method, the device comprising:

• an illuminator configured to illuminate a sample, at least partially, through an asymmetrical first illumination pupil and to illuminate the sample, at least partially, through an asymmetrical second illumination pupil;
• a detector configured to at least partially image the sample through an asymmetrical first detection pupil, in order to generate a first partial image, and to image the sample at least partially through an asymmetrical second detection pupil, in order to generate a second partial image, wherein the first illumination pupil and the first detection pupil are arranged pivoted in relation to one another and partially overlapping one another in projection on a plane perpendicular to an optical axis in such a way that at least one first region of an angular space is in a bright field and one second region of the angular space different from the first region is in a dark field, and the first partial image has a first bright field component and a first dark field component, and wherein the second illumination pupil and the second detection pupil are arranged pivoted in relation to one another and partially overlapping one another in projection on the plane perpendicular to the optical axis in such a way that at least one third region of the angular space is in a bright field and one fourth region of the angular space different from the third region is in a dark field and the second partial image has a second bright field component and a second dark field component, and
• a controller configured to generate an image of the sample from the first partial image and the second partial image.