Method of Determining Disease State Risk
AssigneeLincoln Memorial University
InventorPaul L. Wood
Life of this patent
Estimating the term…
Abstract
A method for determining colorectal cancer risk includes obtaining a blood sample of the subject, isolating serum or EDTA plasma from the blood sample, analyzing the serum or EDTA plasma to determine plasma levels of very long chain dicarboxylic acid (VLCDCA 28:4), comparing the determined plasmas level of VLCDCA 28:4 of the subject with a predetermined range of plasma levels of VLCDCA 28:4 of diagnosed subjects having colorectal cancer, and determining a colorectal cancer risk exists when the determined plasma level of VLCDCA 28:4 is within the predetermined range of plasma levels of VLCDCA.
Claims15
A method of determining disease state risk in a subject for a disease state selected from the group consisting of kidney cancer, colorectal cancer, head and neck cancer, rheumatoid arthritis, and combinations thereof, the method comprising:
- isolating serum or EDTA plasma from a blood sample;
- determining a plasma level of VLCDCA 28:4 from the isolated serum or EDTA plasma;
- comparing the determined plasma level of VLCDCA 28:4 from the isolated serum of EDTA plasma with a predetermined range of VLCDCA 28:4 plasma levels,
- the predetermined range of VLCDCA 28:4 plasma levels previously
- determined from multiple subjects not diagnosed as having the disease state;
- and
- determining the disease state risk exists when the determined plasma level of VLCDCA 28:4 from the isolated serum or EDTA plasma is at least 25% lower than the predetermined range of VLCDCA 28:4 plasma levels.
A method of validating the structure of a 28 to 36 carbon very-long chain dicarboxylic acid, the method comprising
- isolating serum or EDTA plasma from a blood sample;
- storing the serum or EDTA plasma in a low temperature environment;
- mixing about 1 milliliter (mL) of methanol comprising 1 nanomole of [2H28] dicarboxylic acid 16:0 with the isolated serum or EDTA plasma;
- mixing about 1 mL of distilled water and about 2 ml of tert-butyl methylether with the isolated serum or EDTA plasma;
- separating an organic layer from the mixture;
- drying the organic layer;
- dissolving the dried organic layer in a combination of isopropanol, methanol, chloroform, and ammonium acetate;
- performing mass spectrometry on the dissolved organic layer;
- and
- quantifying anions of dicarboxylic acid using negative ion electrospray ionization to validate the dicarboxylic acid 28:4 structure
Dependencies are inferred from the claim text.