Methods of detection and treatment for cardiovascular disease and foot wounds
AssigneeWashington University
InventorsMohamed Zayed, Clay Semenkovich
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Abstract
Among the various aspects of the present disclosure is the provision of a method of detection, treatment, and monitoring of cardiovascular disease or a foot wound by detection of a novel biomarker, Fatty Acid Synthase (FAS). Briefly, therefore, the present disclosure is directed to methods that allow for improved, noninvasive, and reliable diagnosis of these conditions, particularly in subjects suffering from Type 2 Diabetes (T2D).
Claims27
A method of detecting a level of circulating Fatty Acid Synthase (FAS) enzyme content in a subject who has or is suspected of having a cardiovascular-related disease, disorder, or condition, comprising:
- providing a serum or plasma sample from the subject;
- and
- detecting a level of circulating FAS enzyme content using an ELISA assay in the serum or plasma sample,
- wherein the serum or plasma is in an amount sufficient for the level of circulating FAS enzyme content to be detected in an ELISA assay.
A method of diagnosing a cardiovascular-related disease, disorder, or condition comprising:
- providing a serum or plasma sample from a subject suspected of having a cardiovascular-related disease, disorder, or condition;
- detecting a level of circulating FAS enzyme content using an ELISA assay in the serum or plasma sample;
- and
- comparing the level of circulating FAS enzyme content of the subject and a control;
- wherein,
- the subject is diagnosed with a cardiovascular-related disease, disorder, or condition if the level of circulating FAS enzyme content in the subject is elevated compared to the level of circulating FAS enzyme content in a control;
- and
- the serum or plasma is in an amount sufficient for the level of circulating FAS enzyme content to be detected in an ELISA assay.
A method of treating a subject with a foot wound or a cardiovascular-related disease, disorder, or condition, comprising:
- measuring a level of circulating FAS enzyme content in the subject who has or is suspected of having a cardiovascular-related disease, disorder, or condition, comprising:
- providing a serum or plasma sample from the subject;
- and
- detecting a level of circulating FAS enzyme content using an ELISA assay in the serum or plasma sample,
- wherein the serum or plasma is in an amount sufficient for the level of circulating FAS enzyme content to be detected in an ELISA assay;
- and
- treating the subject having the foot wound or the cardiovascular-related disease, disorder, or condition with a vasodilator if the subject has an elevated level of circulating FAS enzyme content compared to a control.
Dependencies are inferred from the claim text.