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US 2022/0034880 A1Published application
High specificity and sensitivity immunosorbent diagnostic assays with simultaneous resolution of multiple antibody isotypes
OwnerIrving L. Weissman, Michal Caspi Talinventors
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Abstract
Compositions and methods are provided for diagnosis of infections. The patterns of antibody isotype, subtype and glycosylation provide for a signature pattern that can identify infective agents and patient response to infection. Patients likely to benefit from therapeutic intervention can be discriminated from patients that have a low probability of responsiveness. Therapies are also provided.
Claims59
A method of characterizing an immune response to a pathogen by an individual, the method comprising:
- collecting at least one antibody-containing sample from the individual;
- contacting said at least one antibody-containing sample from the individual with a diagnostic pathogen;
- contacting the diagnostic pathogen with one or more isotype-specific or glycosylation-specific reagents, which reagents are operably linked to a detectable moiety;
- and
- analyzing the diagnostic pathogen for the presence of bound isotype-specific or glycosylation-specific reagents to determine the presence and type of pathogen-specific antibodies, wherein the presence and type is indicative of a pathogen infection and immune response.
2 more elements
A method of diagnosing and treating an individual with Lyme disease, the method comprising:
- collecting at least one antibody-containing sample from the individual;
- contacting said at least one antibody-containing sample from the individual with a diagnostic bait displaying a plurality of Borrelia burgdorferi pathogen antigens;
- contacting the diagnostic bait with one or more isotype-specific or glycosylation-specific reagents, which reagents are operably linked to a detectable moiety;
- analyzing the diagnostic bait for the presence of bound isotype-specific or glycosylation-specific reagents to determine the presence and type of Borrelia burgdorferi pathogen-specific antibodies, wherein the presence and type is indicative of a Borrelia burgdorferi infection and an immune response to the Borrelia burgdorferi pathogen;
- diagnosing the individual with Lyme disease if the presence of one or more Borrelia burgdorferi pathogen-specific antibodies is detected, and
- treating the individual for Lyme disease if the presence of one or more Borrelia burgdorferi pathogen-specific antibodies is detected, wherein one or more of an antihistamine;
- anti-IgE agent or mast cell stabilizing agent is administered to the individual if the presence of Borrelia burgdorferi pathogen-specific immunoglobulin E (IgE) antibodies is detected.
4 more elements
Dependencies are inferred from the claim text.