Composition comprising material for regulating oct4 modification to repress stemness

A method of regulating stemness through regulation of the function of OCT4 modification in vitro, comprising:

• inhibiting the phosphorylation of serine 351 in the amino acid sequence of mouse octamer-binding transcription factor 4 (OCT4) or serine 359 in the amino acid sequence of human OCT4;
• and
• activating Wnt signaling.

An antibody for detecting whether serine 351 in the amino acid sequence of mouse octamer-binding transcription factor 4 (OCT4) or serine 359 in the amino acid sequence of human OCT4 is phosphorylated,

• wherein the antibody recognizes an epitope represented by an amino acid sequence of SEO ID NO:
• 7 or 8 and specifically binds to phosphorylated serine 351 of mouse OCT4 or phosphorylated serine 359 of human OCT4.

A method of diagnosing whether the stemness of stem cells is repressed, comprising:

• (a) detecting any one or more selected from the group consisting of the phosphorylation of serine 281 in the amino acid sequence of mouse octamer-binding transcription factor 4 (OCT4) or serine 288 in the amino acid sequence of human OCT4, the methylation of arginine 179 in the amino acid sequence of mouse OCT4, arginine 186 in the amino acid sequence of human OCT4, lysine 215 in the amino acid sequence of mouse OCT4, or lysine 222 in the amino acid sequence of human OCT4, and the phosphorylation of serine 351 in the amino acid sequence of mouse OCT4 or serine 359 in the amino acid sequence of human OCT4;
• and
• (b) determining that the stemness of stem cells is repressed when serine 281 in the amino acid sequence of mouse OCT4 or serine 288 in the amino acid sequence of human OCT4 is phosphorylated, when the methylation of arginine 179 in the amino acid sequence of mouse OCT4, arginine 186 in the amino acid sequence of human OCT4, lysine 215 in the amino acid sequence of mouse OCT4, or lysine 222 in the amino acid sequence of human OCT4 is inhibited, or when the phosphorylation of serine 351 in the amino acid sequence of mouse OCT4 or serine 359 in the amino acid sequence of human OCT4 is inhibited.

12-29. (canceled)