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US 2022/0034867 A1Published application
Devices and methods for single cell analysis
AssigneeQuantumcyte, Inc.
InventorsJohn Butler, Bidhan Chaudhuri
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Abstract
The present disclosure provides systems, methods, and devices for the simultaneous determination of a single cell's response to a stimuli and characterization of its cell response. The present disclosure further provides methods for detection of disease state, clinical management of a subject suffering from a disease, drug screening, prediction of drug response, and stands to help direct drug and diagnostic development for the treatment of disease.
Claims16
(canceled)
A method of agent transfer comprising:
- providing a plurality of cells from a biological sample comprising a heterogenous population of cells from a patient positioned on a surface of a first solid substrate at a plurality of cell-philic sites;
- immobilizing a portion of the cells in a hydrogel concentration at their respective cell-philic sites thereby creating a plurality of three-dimensional cell microenvironments comprising the hydrogel composition and the cells, the hydrogel composition of each three-dimensional cell microenvironment being sized and positioned so as to extend upward with a dome shape from the surface of the solid substrate;
- dispending an agent onto an agent transfer device comprising a plurality of agent transfer array elements positioned on a surface of a second solid substrate;
- and
- contacting the plurality of agent transfer array elements to the plurality of three-dimensional cell microenvironments, such that each agent transfer array element corresponds to a three-dimensional cell microenvironment, wherein the contacting allows the agent to be transferred to the plurality of three-dimensional cell microenvironments,
2 more elements
Dependencies are inferred from the claim text.