Systems and methods for meso-dissection
AssigneeRoche Molecular Systems, Inc.
InventorsMichael Barnes, Christophe Chefd'hotel, Srinivas Chukka, Mohammad Qadri
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Abstract
The subject disclosure presents systems and methods for improved meso-dissection of biological specimens and tissue slides including importing one or more reference slides with annotations, using inter-marker registration algorithms to automatically map the annotations to an image of a milling slide, and dissecting the annotated tissue from the selected regions in the milling slide for analysis, while concurrently tracking the data and analysis using unique identifiers such as bar codes.
Claims20
An instrument for meso-dissection, comprising:
- a processor;
- and
- a memory coupled to the processor, the memory to store computer-readable instructions that, when executed by the processor, cause the processor to perform operations comprising:
- importing a reference image along with one or more annotations, wherein the reference image was digitized from a reference slide scanned on a whole-slide scanner and wherein the annotations were generated using a whole slide viewer interface coupled to the whole-slide scanner;
- and
- automatically registering said one or more annotations onto a live capture of a tissue specimen slide to be milled;
- wherein tissue is extracted from the tissue specimen slide is dissected based on the one or more annotations, resulting in a milled tissue sample.
An instrument for meso-dissection, comprising:
- a processor;
- and
- a memory coupled to the processor, the memory to store computer-readable instructions that, when executed by the processor, cause the processor to perform operations comprising:
- obtaining one or more reference annotations derived from one or more reference slides;
- automatically registering the one or more reference annotations onto a milling image of a tissue specimen disposed on a milling slide;
- and
- dissecting one or more portions of tissue from the tissue specimen disposed on the milling slide based on the one or more reference annotations.
A tangible non-transitory computer-readable medium to store computer-readable code that is executed by a processor to perform operations comprising:
- generating one or more milling annotations based on a plurality of reference annotations;
- automatically registering the one or more milling annotations onto a milling image of a tissue specimen disposed on a milling slide;
- and
- milling the tissue specimen using the one or more milling annotations.
Dependencies are inferred from the claim text.