Method for detecting and quantifying latent retroviral rna species
AssigneeBio-Rad Laboratories, Inc.
InventorsMichael L. Samuels, Darren R. Link, Sirshendu Roopom Banerjee
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Abstract
The invention includes methods for determining the presence of a latent viral population by analyzing an RNA population from the virus with digital techniques, such as digital PCR or by sequencing cDNA produced from the RNA. The invention additional includes methods for determining the presence of latent viral populations by detecting and/or quantifying enzymes that are uniquely associated with the virus, e.g., reverse transcriptases.
Claims20
A method for detection of latent retrovirus, comprising the steps of:
- isolating a virion particle in an aqueous droplet surrounded by an immiscible fluid;
- lysing the virion particle in the aqueous droplet to release an RNA molecule;
- exposing the RNA molecule to at least one primer capable of hybridizing to at least a portion of the RNA molecule;
- synthesizing a cDNA product from the RNA molecule;
- and
- detecting the cDNA product.
A method for detecting a latent retrovirus species, the method comprising the steps of:
- isolating virion RNA in an aqueous droplet surrounded by an immiscible fluid,
- exposing the virion RNA in the droplet to at least one primer capable of hybridizing to at least a portion of the virion RNA;
- synthesizing a cDNA product from the virion RNA;
- and
- detecting the cDNA product.
Dependencies are inferred from the claim text.