Back to results
US 2022/0033895 A1Published application
Linked paired strand sequencing
AssigneeSingular Genomics Systems, Inc.
InventorsEli N. Glezer, Martin Maria Fabani, Timothy Looney, Sabrina Shore, Ryan Shultzaberger, Daan Witters
Life of this patent
Estimating the term…
Abstract
Provided herein are methods for sequencing both strands of a double stranded nucleic acid fragment that improves fidelity and accuracy of a sequence determination compared to traditional next generation sequencing methods. Compositions and kits for use in the methods are also provided.
Claims23
-47. (canceled)
A method of selectively sequencing a double-stranded nucleic acid, the method comprising:
- (a) ligating a first adapter to a first end of the double-stranded nucleic acid, and ligating a second adapter to a second end of the double-stranded nucleic acid, wherein the second adapter is a hairpin adapter;
- (b) displacing at least a portion of one strand of the double-stranded nucleic acid from step (a);
- (c) hybridizing a probe oligonucleotide to the displaced portion of the double-stranded nucleic acid;
- (d) separating the probe-hybridized double-stranded nucleic acid from nucleic acids not hybridized to a probe;
- and
- (e) sequencing the probe-hybridized double-stranded nucleic acid of step (d).
3 more elements
-56. (canceled)
-84. (canceled)
Dependencies are inferred from the claim text.