Back to results
US 2022/0033892 A1Published application
REAGENTS FOR DETECTING ALU ELEMENTS IN CELL-FREE DNA (cfDNA)
AssigneeThe Regents of the University of California
InventorsMinnie M. Sarwal, Tara K. Sigdel, Joshua Y. Yang
Life of this patent
Estimating the term…
Abstract
Provided herein is the use of measurements of cell-free DNA, protein, and/or metabolite found in biofluid (e.g., urine) for identifying and treating organ injury. Provided herein are methods and compositions for monitoring, detecting, quantifying, and treating kidney injury in subjects suffering from or suspected of having an altered renal status by measuring amounts of cfDNA and one or more other markers, such as inflammation markers, apoptosis markers, protein, and DNA methylation.
Claims22
A reaction mixture comprising:
- i) non-amplified cell-free DNA (cfDNA) extracted from a urine sample;
- and
- ii) a nucleic acid probe having a 3′ end and a 5′ end, wherein the nucleic acid probe is complementary to at least 20 contiguous nucleotides of SEQ ID NO:1 and wherein the 3′ end and/or the 5′ end is covalently ligated to at least one detectable moiety.
Dependencies are inferred from the claim text.