Engineered biosynthetic pathways for production of 2-oxoadipate by fermentation
AssigneeZymergen Inc.
InventorsAnupam Chowdhury, Steven M. Edgar, Alexander Glennon Shearer, Cara Ann Tracewell, Stepan Tymoshenko, Zhihao Wang
Life of this patent
Estimating the term…
Abstract
The present disclosure describes the engineering of microbial cells for fermentative production of 2-oxoadipate and provides novel engineered microbial cells and cultures, as well as related 2-oxoadipate production methods.
Claims60
An engineered microbial cell that expresses a heterologous homocitrate synthase, wherein the engineered microbial cell produces 2-oxoadipate.
An engineered microbial cell that expresses a non-native homocitrate synthase, wherein the engineered microbial cell produces 2-oxoadipate.
An engineered microbial cell, wherein the engineered microbial cell comprises means for expressing a heterologous homocitrate synthase, wherein the engineered microbial cell produces 2-oxoadipate.
The engineered microbial cell of claim 14 or claim 18, wherein the engineered microbial cell also comprises means for expressing a non-native homoisocitrate dehydrogenase.
An engineered microbial cell, wherein the engineered microbial cell comprises means for expressing a non-native homocitrate synthase, wherein the engineered microbial cell produces 2-oxoadipate.
A method for preparing 2-oxoadipate using microbial cells engineered to produce 2-oxoadipate, the method comprising:
- (a) expressing a non-native homocitrate synthase in microbial cells;
- (b) cultivating the microbial cells in a suitable culture medium under conditions that permit the microbial cells to produce 2-oxoadipate, wherein the 2-oxoadipate is released into the culture medium;
- and
- (c) isolating 2-oxoadipate from the culture medium.
Dependencies are inferred from the claim text.