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US 2022/0033854 A1Published application
Systems and methods for expressing proteins
AssigneeCorning Incorporated
InventorsNa Li, Jie Wang
Life of this patent
Estimating the term…
Abstract
The present disclosure relates to method and compositions for generating proteins. In particular, the present disclosure relates to electroporation mediated gene delivery in the generation of recombinant proteins (e.g., drug metabolizing enzyme and transporter vesicles) in mammalian cells.
Claims19
A method of screening a test compound, comprising:
- a) contacting a mammalian cell with a nucleic acid encoding a transporter protein selected from the group consisting of ABCB1, ABCB4, ABCB11, ABCC1, ABCC2, ABCC3, ABCC4, ABCC5, ABCC6, ABCG2, and a homolog thereof;
- b) electroporating said mammalian cell such that said nucleic acid enters said mammalian cell;
- c) isolating cell membranes expressing said transporter protein;
- and
- d) contacting said cell membranes with said test compound.
2 more elements
A method of generating a cell fraction containing a protein of interest, comprising:
- a) contacting a mammalian cell with a nucleic acid encoding a drug metabolizing enzyme selected from the group consisting of a cytochromes P450, an aldehyde oxidase (AO), a flavin monooxygenase (FMO), a monoamine oxidase A and B (MAO A and B), an esterase, a N-acetyltransferase (NAT), a sulfotransferase (SULT), a uridine 5′-diphospho-glucuronosyltransferase (UGT), and a homolog thereof;
- b) electroporating said mammalian cell such that said nucleic acid enters said mammalian cell;
- and
- c) isolating a cell fraction containing said drug metabolizing enzyme.
1 more element
Dependencies are inferred from the claim text.