Brassica event mon94100 and methods of use thereof
AssigneeMonsanto Technology LLC
InventorsChristine M. Ellis, Shirley X. Guo, Sherry LeClere, Mingsheng Peng, Janice R. Weihe
Life of this patent
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Abstract
The invention provides recombinant DNA molecules that are unique to Brassica Event MON94100 and transgenic Brassica plants, Brassica plant parts, Brassica seeds, Brassica cells, and agricultural products containing Brassica Event MON94100 as well as methods of using and detecting Brassica Event MON94100. Transgenic Brassica plants containing Brassica Event MON94100 exhibit tolerance to dicamba.
Claims26
A recombinant DNA molecule comprising a sequence selected from the group consisting of SEQ ID NO:10, SEQ ID NO:9, SEQ ID NO:8, SEQ ID NO:7, SEQ ID NO:6, SEQ ID NO:5, SEQ ID NO:4, SEQ ID NO:3, SEQ ID NO:2, and SEQ ID NO:1.
3-4. (canceled)
A DNA molecule having a sufficient length of contiguous nucleotides of SEQ ID NO:10 to function as a DNA probe specific for SEQ ID NO:10 in a sample of DNA derived from a Brassica plant, Brassica seed, or Brassica cell.
A pair of DNA molecules comprising a first DNA molecule and a second DNA molecule, wherein the first and second DNA molecules each comprise a fragment of SEQ ID NO:10 and function as DNA primers when used together in an amplification reaction with DNA containing Brassica Event MON94100 to produce an amplicon diagnostic for Brassica Event MON94100 in a sample.
A method of detecting the presence of Brassica Event MON94100 in a sample derived from a Brassica plant, Brassica seed, or Brassica cell, the method comprising:
- a) contacting the sample with at least one antibody specific for at least one protein encoded by Brassica Event MON94100;
- and
- b) detecting binding of the antibody to the protein in the sample,
- wherein the binding of the antibody to the protein indicates the presence of Brassica Event MON94100 in the sample.
A kit for detecting the presence of Brassica Event MON94100 comprising a DNA probe specific for SEQ ID NO:10, a pair of DNA primers that produce an amplicon diagnostic for Brassica Event MON94100, or an antibody specific for at least one protein encoded by Brassica Event MON94100.
(canceled)
A method of improving tolerance to dicamba in a Brassica plant comprising:
- a) obtaining a DNA construct comprising in operable linkage (a) a promoter from Peanut chlorotic streak virus, (b) a leader from Tobacco etch virus, (c) a ribulose 1,5-bisphosphate carboxylase chloroplast transit peptide from Pisum sativum, (d) a dicamba monooxygenase coding sequence from Stenotrophomonas maltophilia, and (e) a 3′ UTR from Medicago truncatula;
- and
- b) inserting the DNA construct into the genome of a Brassica cell;
- c) regenerating the Brassica cell into a Brassica plant;
- and
- d) selecting a Brassica plant comprising the DNA construct.
Dependencies are inferred from the claim text.